. Block diagram of an HPLC–MS. A 3 ingredient mixture enters the HPLC. When part A elutes from your column, it enters the MS ion supply and ionizes to sort the dad or mum ion and several other fragment ions.. HPLC separation of a mix of flavonoids with UV/Vis detection at 360 nm and, from the inset, at 260 nm. The selection of wavelength has an e
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